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11.
12.
光合细菌在对虾育苗生产中的应用 总被引:6,自引:1,他引:6
1995年4月24日~5月8日,在文登高岛盐场育苗场50m3水体育苗池,进行了用几株光合细菌(RhodopseudomonasS.P.)混合菌液作为中国对虾(Penaeuschinensis)苗期水质净化剂及辅助饵料,取得了明显效果。育苗池水透明度提高约20cm,幼体挂脏率下降50%,变态提早1d左右,且多产仔虾百万尾 相似文献
13.
两株琼胶酶高产细菌的筛选和鉴定 总被引:4,自引:1,他引:4
从海洋环境中筛选得到两株琼胶酶的高产菌株,通过形态观察和生理生化反应,确定它们属于弧菌属,通过BIOLOG细菌鉴定系统鉴定,并同弧菌属标准菌株分析比较,确定上方宝剑两株菌都是塔式弧菌(Vibrio tubiashii),这两株菌在碳源利用方面存在差别。 相似文献
14.
Akira Shibata Kouichi Ohwada Masakazu Tsuchiya Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97
Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate
organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted
for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present
study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater
cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size,
0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and
correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG
to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m,
which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell−1 obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29)
and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest
that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas
this fraction was reduced in the middle layer. 相似文献
15.
阐述把维生素B12生产菌添加到培养水中培养褶皱臂尾轮虫BrachionusPlicatilis的买验。共18株细菌分离于轮虫培养池,其中,有一株产维生素B12的假单胞杆菌TP4对轮虫的生长繁殖有明显的促进作用。把TP4菌株培养后,加入到2L的烧杯和500L的水槽中培养泰国S型轮虫时.在9d(天)和6d(天)中,轮虫密度从124~139和242~288个体/ml增殖到4,417~5,540和1,017~1,254个体/ml,分别比对照组增加了4~6及2~3倍。 相似文献
16.
Young-Ok Kim Eun Jin Yang Jung-Hoon Kang Kyoungsoon Shin Man Chang Cheol Soo Myung 《Ocean Science Journal》2007,42(1):9-17
The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and
heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake
Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner
area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L1) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher
densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while
microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed
into more than 106 cells L1 at the surface layer of the inner area, while its abundance was much lower (103-104 cells L1) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p <
0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing
of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates. 相似文献
17.
2002年2月—2004年11月对胶州湾异养细菌和大肠菌群进行长期调查发现,胶州湾异养细菌丰度在105—106cells/ml之间,其中河口区域和近岸区域数量较高,其它区域相对较低。从季节上来看,夏季、秋季细菌丰度较高(分别为7.4×105、7.2×105cells/ml),冬季和春季丰度较低(分别为4.1×105cells/ml、5.9×105cells/ml),且在一年内呈现出一定的规律性波动。从季节尺度上看异养细菌丰度与温度呈现很好的正相关性(R=0.54)。对大肠菌群的研究表明,河口区域的大肠菌群数量显著高于其它区域,并且河口区域大肠菌群常年处于严重超标状态(最高为1.1×106cells/L,超过国家前三类水质标准的100倍)。根据大肠菌群的数量状况可以将胶州湾分为三个区域,即严重污染区、中度污染区和轻度污染区。该区域划分与通过营养盐对胶州湾区域的划分(富营养区、中度营养区、贫营养区)结果一致。 相似文献
18.
Microbial Biomass Dynamics Along a Trophic Gradient at the Atlantic Barrier Reef off Belize (Central America) 总被引:2,自引:0,他引:2
Abstract. Recent findings indicate that heterotrophic bacteria and not phytoplankton are the most numerous biomass components even in the euphotic zone of oligotrophic, open oceans. In this study it was hypothesized that the microbial biomass components change within a few hundred meters as oligotrophic water flows across the reef and becomes enriched with nutrients. Along a trophic gradient, four stations at the Atlantic Barrier Reef off Belize (Central America) were sampled for microbial biomass components. Phytoplankton biomass (measured as chlorophyll a) ranged from the most oligotrophic station (St. 1) to the most eutrophic station (St. 4) from 6.9–415.5 μg CI"' (assuming a C:chl a ratio of 30): heterotrophic bacterial biomass increased 4-fold (from 10.1–46.4μg C 1-1 ), heterotrophic nanoflagellate (HNAN) biomass increased from 4.6-19ug C 1-1 , and cyanobacteria from 0.9-4.5 μg C-1-1 . Production estimates derived from seawater cultures revealed a 5-fold increase in bacterial production from the oligotrophic station (3.7 ug C 1-1 d-1 ) to the eutrophic St. 4 (17.8ug C-1-d1-1 )- Cyanobacterial production rose from 1.1–3.5ug C-1–d-1 and HNAN production from 0.65-1.13 μg C-1-1 -d-1 . While cyanobacteria contributed between 13 and 20% to the autotrophic plankton component in the oligotrophic waters, their contribution dropped to about 1 % at the eutrophic stations. 相似文献
19.
20.
Koji Suzuki Akira Hinuma Hiroaki Saito Hiroshi Kiyosawa Hongbin Liu Toshiro Saino Atsushi Tsuda 《Progress in Oceanography》2005,64(2-4):167
To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l−1 in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l−1 in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l−1 at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment. 相似文献